Abstract
To evaluate the effects of the topical liver hypothermia and IPC combination against I/R injury after initial reperfusion. In 32 Wistar rats, partial liver ischemia was induced for 90 minutes in normothermia (IN), ischemic preconditioning (IPC), 26ºC topical hypothermia (H) and 26ºC topical hypothermia plus IPC (H+IPC). MAP, body temperature and bile flow were recorded each 15 minutes. Plasmatic injury markers and tissue antioxidant defenses were assessed after 120 minutes of reperfusion. MAP and body temperature remained constant during all experiment. Bile flow returned to levels similar to controls after 45 minutes of reperfusion in the H and H+IPC groups and increased significantly in comparison to the NI and IPC groups after 105 and 120 minutes. AST and ALT increased significantly in the normothermic groups in comparison to controls. TBARS levels decreased significantly in the H+IPC group in comparison to the other groups whereas Catalase levels increased significantly in the IPC group. SOD levels were significantly higher in the H group in comparison to all groups. The induction of 26ºC topical hypothermia associated or not to IPC protected the ischemic liver against ischemia/reperfusion injuries and allowed an early recovery of the hepatic function.
Highlights
Ischemia-reperfusion injury (I/R) is a major cause of morbidity and mortality in liver surgery and transplantation
The protective effect of hypothermia has been attributed to a reduction of liver metabolism and the subsequent decrease of oxygen requirement that occurs with a temperature decrease during the ischemia[3]
The Mean Artery Pressure (MAP) and body temperature remained constant over the whole experiment
Summary
Ischemia-reperfusion injury (I/R) is a major cause of morbidity and mortality in liver surgery and transplantation. In order to attenuate the deleterious effects of I/R, some strategies such as topical Hypothermia and IPC have been used with proven benefits[1,2]. The protective effect of hypothermia has been attributed to a reduction of liver metabolism and the subsequent decrease of oxygen requirement that occurs with a temperature decrease during the ischemia[3]. The prevention of liver injuries promoted by attenuation of oxidative stress and microcirculatory injury after the reperfusion plays an important role[3,4,5]. In the present study the authors assessed the occurrence of protective synergistic effects when both techniques are associated as well as the hepatocelular injuries, liver function and antioxidant defenses at the initial phase of the reperfusion
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