Abstract
Considering the increase in incidence of multidrug resistance (MDR) in yeast, there is, in parallel, spurt in research in the field. Expectedly there are several good articles which highlight the MDR research, but there is very limited information available which deals with the methods used to study MDR in yeast. In this contribution, an attempt is made to describe in detail routine protocols used to study different aspects of MDR in yeast, particularly dealing with multidrug efflux pump protein-related functions. Different drug susceptibility assays can be performed on solid or on liquid media using clinically approved protocols. Moreover, properties and activity scales of drug efflux pumps can be measured by performing their substrate efflux activity using suitable substrate depending on choice of protocol or transporter under study. These drug transporters can be overexpressed in different systems or can be expressed in drug-susceptible strains to clearly follow their contribution in development of drug resistance. It is hoped that this chapter will be useful in studying the role of drug transporters in the phenomenona of MDR in yeast.
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