Abstract
Summary Background: Secretory component (SC) is the extracellular component of the polymeric immunoglobulin receptor (pIgR) that functions as a receptor for externally secreted polymeric immunoglobulins such as secretory IgA. SC expression is regulated by a signaling pathway involving TNF-a. Methods: Caco-2 cells were cotreated with TNF-a plus either Wortmannin, which inhibits PI3K, or Rapamycin, which inhibits mTOR. The expression of SC and pIgR mRNA were assessed by immunocytochemistry, Western blotting, and Quantitative real-time PCR. Results: Wortmannin and Rapamycin decreased the fraction of cells expressing SC, as well as the total expression of SC protein and pIgR mRNA. Conclusions: TNF-a regulation of SC expression is mediated through a PI3K/AKT/mTOR signaling pathway in Caco-2 cells.
Highlights
Secretory component (SC) is the extracellular component of the polymeric Ig receptor which functions as a receptor for polymeric immunoglobulin that is secreted into external fluids as secretoryAddress for correspondence: Dongyan Liu, Ph.D., MD China Medical University Affiliated Shengjing Hospital Research Center and Key Laboratory of Congenital Malformation Research, Ministry of Health, No 36 Sanhao Street, Heping District, Shenyang City, Liaoning Province, China, 110004 e-mail: Dongyan.Liuayahoo.com.cn immunoglobulin A (SIgA)
Secretory component (SC) is the extracellular component of the polymeric immunoglobulin receptor that functions as a receptor for externally secreted polymeric immunoglobulins such as secretory IgA
The expression of SC and polymeric immunoglobulin receptor (pIgR) mRNA were assessed by immunocytochemistry, Western blotting, and Quantitative real-time PCR
Summary
Address for correspondence: Dongyan Liu, Ph.D., MD China Medical University Affiliated Shengjing Hospital Research Center and Key Laboratory of Congenital Malformation Research, Ministry of Health, No 36 Sanhao Street, Heping District, Shenyang City, Liaoning Province, China, 110004 e-mail: Dongyan.Liuayahoo.com.cn immunoglobulin A (SIgA). SIgA is the most abundant intestinal immunoglobulin, as well as the first line of specific immunological defense against environmental antigens. SC is a nonspecific scavenger of microorganisms and plays a key role in protecting and balancing the intestinal mucous membrane in limiting the inflammation process [1]. SC is a key defense to bacteria [2,3,4,5] and parasites [6]. Tumour necrosis factor alpha (TNFa) is a well-known member of the TNF superfamily consisting of at least 18 ligands and 29 different receptors involved in numerous cellular processes.
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