Abstract
A rapid, sensitive and accurate TLC method has been developed for estimation of various pharmaceutical agents, such as piroxicam, tenoxicam, celecoxib and rofecoxib in human whole blood and urine. TLC analysis was performed on silica gel F254 plates with ethyl acetate-toluene-butylamine (2 : 2 : 1, v/v/v) and chloroform-acetone-toluene (6 : 2.5 : 1, v/v/v) as mobile phases. Densitometric scanning was performed at 254, 301 and 370 nm. The method was validated in terms of linearity, accuracy, precision, sensitivity and selectivity. The response was a linear function of concentration in the range of 1.4–39.1 μg/mL (r = 0.99). Precision of the assay was in the range 0.5–1.8%, and average accuracy was close to 100%. Presented method can be applied to routine quantification studies of chosen drugs in biological material.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
