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This is an accepted article with a DOI pre-assigned that is not yet published.M. melanocarpa is a tall climber, belonging to the family Fabaceae. Mucuna melanocarpa is an endemic plant to Ethiopia. While the genus Mucuna is known for its nutritional (e.g., Mucuna pruriens) and medicinal properties, the understudied species Mucuna melanocarpa remains a largely unexplored reservoir of unique bioactive compounds. Therefore, this study aimed to investigate the qualitative and quantitative phytochemical analysis and bioactive compound identification using GC-MS technique. 50 g of the sample powder was added into a round beaker containing 500 mL of ethanol and then kept in a rotary shaker at 190-220 rpm for 48 hrs at 37℃ and the solution was filtered using Whatman filter paper No. 1. The filtrate was evaporated at room temperature to get the dry extract. The GC-MS analysis of M. melanocarpa seed extract was performed using GC -MS equipment to identify the phytochemical constituents. The phytochemical screening revealed the presence of alkaloids, flavonoids, steroids, terpenoids saponins, tannins and proteins. The GC-MS analysis showed the presence of two major compounds Octacosane (10.45%) and Geraniol (3.02%) known for their Antimicrobial, antioxidant, anti-inflammatory and anti-tumor property.