Titanium Surfaces Modified with Graphene Oxide/Gelatin Composite Coatings for Enhanced Antibacterial Properties and Biological Activities

Abstract

Titanium alloys have been widely used in orthopedic implants due to their excellent physicochemical properties and good biocompatibility. However, in practice, titanium implants may fail to integrate or develop an implant-centered infection. Because of its excellent mechanical properties, bone integrability, biocompatibility, antibacterial properties, and so on, graphene oxide is increasingly being used in the preparation of composite biomaterials. The percutaneous titanium implants are used as the research object in this project. To solve the integration of implant and tissue, a graphene oxide/gelatin (GO/gel) composite coating was used to optimize the implant surface. Bacterial and cell experiments were used to investigate the antimicrobial activity, biocompatibility, and regulation of macrophage polarization of GO/gel-modified titanium. According to our findings, GO/gel-modified titanium has a good bacteriostatic effect against Staphylococcus aureus. On the modified surface, L929 cells proliferated well and showed no cytotoxicity. Simultaneously, the GO/gel-modified titanium surface could inhibit macrophage adhesion and spread in the early stage of culture and showed a more obvious inflammatory decline in the late stage of culture. These findings implied that GO/gel-modified titanium is advantageous for resistant bacteria and tissue remolding.