Time of ovulation in the South Australian Merino ewe following synchronization of estrus. 1. Variation within and between flocks

Abstract

Observations were made by repeated laparoscopy to determine the time of ovulation in seven flocks of South Australian Merino ewes treated with pregnant mare serum gonadotropin (PMSG, 400 IU per ewe) and an intravaginal pessary containing 60 mg medroxy-progesterone acetate (MAP). Ovulation most often commenced within 57 h of pessary removal and was completed in all flocks within 81 h. There was, however, significant variation in the time of onset of ovulation; it did not commence until 69 h in one of three observations of Flock 1 and in two of three observations of Flock 2. On the other hand, in a flock of two-year-old nulliparous ewes (Flock 7), ovulation commenced significantly (P < 0.001) earlier than in most observations of other flocks, with 18 39 ewes ovulating within 57 h of pessary removal. The effect of the progestagen (MAP vs flugestone acetate) and the gonadotropin preparation (Pregnecol vs Folligon) on the time of ovulation were observed in separate studies. The characteristic time of ovulation was not influenced in either comparison. However, ovulation occurred significantly (P < 0.001) earlier in ewes treated with progesterone released from a controlled internal drug release dispenser (CIDR). The implications of these findings to artificial insemination and embryo transfer are discussed.