Abstract
The Phytophtora root and stem rot is a serious disease in soybean. It is caused by the oomycete pathogen Phytophthora sojae. Growing Phytophthora resistant cultivars is the major method of controlling this disease. Resistance is race- or gene-specific; a single gene confers immunity against only a subset of the P. sojae isolates. Unfortunately, rapid evolution of new Phytophthora sojae virulent pathotypes limits the effectiveness of an Rps (“resistance to Phytophthora sojae”) gene to 8–15 years. The current study was designed to investigate the effectiveness of Rps12 against a set of P. sojae isolates using recombinant inbred lines (RILs) that contain recombination break points in the Rps12 region. Our study revealed a unique Rps gene linked to the Rps12 locus. We named this novel gene as Rps13 that confers resistance against P. sojae isolate V13, which is virulent to recombinants that contains Rps12 but lack Rps13. The genetic distance between the two Rps genes is 4 cM. Our study revealed that two tightly linked functional Rps genes with distinct race-specificity provide broad-spectrum resistance in soybean. We report here the molecular markers for incorporating the broad-spectrum Phytophthora resistance conferred by the two Rps genes in commercial soybean cultivars.
Highlights
Soybean [Glycine max (L.) Merr.] is one of the main oilseed crops produced and consumed worldwide and is among the world’s five utmost significant food c rops[1]
To investigate the utility of Rps[12] against a set of P. sojae isolates collected from Iowa soybean field, we looked for recombinant inbred lines (RILs) that carry only Rps[12]
We have investigated 60 Phytophthora resistant RILs generated from the cross between PI399036 × A R217 for simple sequence repeat (SSR) markers linked to the known Rps regions as described below
Summary
Soybean [Glycine max (L.) Merr.] is one of the main oilseed crops produced and consumed worldwide and is among the world’s five utmost significant food c rops[1]. Oomycete pathogens are challenging to control and most fungicides are ineffective because the P. sojae infected roots are difficult to treat effectively with chemicals. The most effective way to reduce PRS damage is planting Phytophthora resistant soybean cultivars[11]. Over 200 known pathotypes of this pathogen have been reported and the number is ever growing presumably due to selection pressure on the P. sojae population for new pathotypes that can overcome the newly introduced Rps genes. To determine the utility of Rps[12], we investigated the responses of recombinant inbred lines (RILs) containing Rps[12] against a collection of P. sojae isolates. The Rps[12] and Rps[13] genes together provide broad-spectrum Phytophthora resistance in soybean
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