Ti3C2TxMXene modified indium tin oxide (ITO) electrode for electrochemical sensing of bilirubin based on a molecularly imprinted pyrrole polymer

Abstract

In this study for the first time we are developing Ti3C2TxMXene-based molecularly imprinted electrochemical sensor for the detection of bilirubin (BR). Firstly, Ti3C2TxMXene is synthesized by chemical etching and deposited at the ITO electrode surface by drop casting. After that, pyrrole as a monomer is electropolymerized in the presence of a sodium acetate buffer solution containing the BR template to prepare a BR-imprinted electrode. The modification of ITO electrode with Ti3C2TxMXene has not only imparted binding properties to the substrate that are very important for MIP formation but has also rendered excellent electrochemical characteristics to the electrode as monitored by CV, DPV, and EIS techniques. The sensing studies have been performed with synthetic samples that reveal the potential of prepared molecular imprinted electrochemical sensors toward BR detection. The linearity range, limit of detection, and limit of quantification are calculated as 10 μM to 90 μM, 0.197 μM and 0.598 μM, respectively. Selectivity, stability, and reproducibility are also reported for the prepared MIP sensor.