Thyroid hormones in human tumoral and normal nervous tissues

Abstract

We have studied T4 and T3 concentrations, DNA and protein concentrations and 5′ and 5 deiodinases in samples of brain tumors obtained at surgery from 49 patients, and, in most cases, also from surrounding normal tissue. T4 concentrations in normal cortical tissue (6.19±0.45 ng/g) were lower than in white matter, but the difference disappeared when referred to the DNA content (2.26±0.27 ng/mg DNA). No other differences were found between cortical and white matter, or among cortical lobes. T4 in normal tissue was higher than previously reported, mostly from autopsy samples, whereas T3 (0.99±0.07 ng/g) was similar. 5′D-I activity was negligible as compared to 5′D-II (8.11±1.09 fmol/h/mg protein). When expressed in relation to the different DNA contents of normal vs. tumoral tissue, 5′D-II activities were the same for both. 5D activity was highly variable in the tumoral tissue, with negligible activities in meningiomas and pituitary adenomas. When referred to the DNA content, T4 and 5′D-II were the same, but T3 concentrations were lower in the tumor (0.24±0.03 ng/mg DNA) as compared to normal (0.35±0.04 ng/mg DNA) tissue samples. Whether or not this decrease of T3 affects the expression of T3-sensitive processes remains to be studied.