Abstract

Abstract Introduction: Fine needle aspiration (FNA) biopsy is used for evaluation of thyroid nodules. Overall accuracy exceeds 95% with a false negative rate between 0% and 3%.1,2 Excellent thyroid FNA smear preparation is critical as it facilitates accurate cytologic interpretation by the pathologist.3–5 Materials and Methods: Standard frosted glass slides and hand lotion were used for this demonstration. In the following video we present two techniques: a three-slide technique and a two-slide technique. 1. The “classic” technique uses three slides and generates two slides for interpretation. A slide to be deposited in fixative (a), a slide to be air dried (b), and a spreader slide (c), which is typically discarded. Technique: • Place a small drop of material on the top one-third and the center of the slide (a). • Pick up the slide (a) from the edges and using your nondominant hand pinch the frosted end between your thumb and index finger and use the remaining fingertips of this hand to support the back ...

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