Abstract

BackgroundToday, thrombosis is one of the most widely occurring diseases in modern life. Drugs with thrombolytic functions are the most effective methods in the treatment of thrombosis. Among them, Douchi fibrinolytic enzyme (DFE) is a promising agent. DFE was isolated from Douchi, a typical and popular soybean-fermented food in China, and it can dissolve fibrin directly and efficiently. A strain, Bacillus subtilis LD-8547 produced DFE with high fibrinolytic activity has been isolated in our lab previously.ResultsIn the study, thrombolytic effect of DFE from Bacillus subtilis LD-8547 was studied in vitro and in vivo systematically. The results showed that DFE played a significant role in thrombolysis and anticoagulation in vitro. And the thrombolytic effects correlated with DFE in a dose-dependent manner. In vivo, the acute toxicity assay showed that DFE had no obvious acute toxicity to mice. Test of carrageenan-induced thrombosis in mice indicated that the DFE significantly prevented tail thrombosis, and arterial thrombosis model test indicated that Douchi fibrinolytic enzyme DFE had thrombolytic effect on carotid thrombosis of rabbits in vivo. Other results in vivo indicated that DFE could increase bleeding and clotting time obviously.ConclusionsThe DFE isolated from Bacillus subtilis LD-8547 has obvious thrombolytic effects in vitro and in vivo. This function demonstrates that this enzyme can be a useful tool for preventing and treating clinical thrombus.

Highlights

  • Today, thrombosis is one of the most widely occurring diseases in modern life

  • Purification of Douchi fibrinolytic enzyme (DFE) and fibrinolytic activity assay DFE was purified from Bacillus subtilis LD-8547 with a series of procedures

  • The results showed that the fibrinolytic activity of DFE reached 21750 U/mL after condensed (Figure 1)

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Summary

Introduction

Thrombosis is one of the most widely occurring diseases in modern life. Drugs with thrombolytic functions are the most effective methods in the treatment of thrombosis. A strain, Bacillus subtilis LD-8547 produced DFE with high fibrinolytic activity has been isolated in our lab previously. Among all kinds of cardiovascular diseases, thrombosis is a frequently occurred symptom [1]. The main drugs are urokinase (UK), streptokinase (SK) and tissue plasminogen activator (t-PA). They are too expensive and their half-life is short. They are just fit for injection and have the side effect of hemorrhage.

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