Three New Sensitive Spectrophotometric Methods for the Assay of Non UV Absorbing Tioconazole

Abstract

Abstract Three simple, sensitive and accurate spectrophotometric methods for the determination of non UV absorbing tioconazole in pure form and in pharmaceutical formulations are described. The first method depends upon measuring the second derivative (D2) peak amplitude of tioconazole in 0.1 N hydrochloric acid at 248 nm. The second method involves the reaction of tioconazole with picric acid in chloroform and measuring the first derivative (D1) peak amplitude at 422 nm or the peak trough amplitude at 360-325 nm. The third method is based on the reaction of tioconazole with mercurochrome in methanol to give an intense red colored chromogen measurable at 547 nm. The optimization of different experimental conditions is described. Beer's law is valid over concentrations ranging from 0.4-2 mg%. The methods have been applied successfully to the analysis of dosage forms.