Analytica Chimica Acta | VOL. 1237
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Three-fluorescence sensor for minute-time scale low-cost analysis of urinary oxalate in urolithiasis metabolic assessment

Publication Date Jan 1, 2023

Abstract

Since oxalate plays an important role in the metabolic assessment of urolithiasis, there is need for convenient and efficient methods for oxalate detection. Herein, we report a three-signal fluorescence strategy for oxalate analysis based on the ability of oxalate to reduce Cu2+ to Cu+, and the ability of pyrophosphate-cerium coordination polymeric networks (PPi-Ce CPNs), cadmium telluride quantum dots (CdTe QDs), and N-Methyl Mesoporphyrin (NMM) to selectively detect Cu2+ and Cu+. The detection range was 100nM to 1mM, the turnaround time was 6min, while the limits of detections for PPi-Ce CPNs, QDs and NMM as reporters were 25nM, 10nM and 40nM, respectively. Visual detection of oxalate relied on color change in the solution, which could be observed using the naked eye. The fluorescent system was used for oxalate analysis in 44 urine samples (32 calcium oxalate stone patients, 12 controls without urolithiasis), and the results were consistent with clinical diagnosis and imaging data. Moreover, the visual system was used to analyze 8 urine samples (4 patients and 4 controls), and showed good consistency with clinical diagnosis and computed tomography imaging results. These findings suggest that the method has potential application for the metabolic assessment of urolithiasis.

Concepts

N-Methyl Mesoporphyrin Cadmium Telluride Quantum Dots CdTe QDs Clinical Diagnosis Turnaround Time Naked Eye Efficient Methods For Detection Detection Range Color Change Clinical Imaging Data

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