Abstract

Some general remarks are made concerning the difficulties experienced in the quantitative determination of the composition of complex sugar mixtures in solution. The application of thin-layer chromatography to this analytical problem is discussed. A technique for obtaining a satisfactory separation of nine to ten sugars has been worked out. Methods of determining reducing sugars and keto-sugars in amounts of 0 to 100 μg are mentioned, as well as a technique for removing the sugar-containing area from the thin-layer plate. Figures illustrating the quantitative aspects of the method are given.

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