Abstract
Since the introduction of vitreous carbon microtome knives progress has been made on improving the quality of the cutting edge. It has been found that long uniform edges produced by mechanical lapping are superior to cleaved edges. Knife edges of 12.5mm to 25mm with 45° cutting angles have been produced, mounted for ultramicrotomes in aluminum boats (Fig. 1) and alpha tested under routine microtomy conditions. In tests at independent laboratories, the 25mm, 45° knives were found to produce routine l-2μm thick sections of a variety of materials embedded in a range of media.Vitreous carbon was commercially obtained in square sheets, 76mm X 76mm X 3mm, cut and processed for polishing by standard machine grinding. Uniform edges with 45° cutting angels were produced by lapidary procedures incorporating the sequential use of fine polishing grits.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
