Abstract
碱性嗜热过氧化氢酶是一种重要的纺织用酶.根据大肠杆菌密码子使用偏爱性,对Thermus thermophilus HB27来源的含锰过氧化氢酶基因进行密码子优化,将优化后的基因连接至表达载体pET28a（＋）上,转化到E.coli BL21（DE3）中进行诱导表达.结果表明在含有14mmol/L Mn2＋浓度的培养液中以0.2 mmol/L的IPTG 42℃条件下诱导2h的情况下,菌体破碎上清液中的酶活力可达25 U/ml.利用Ni亲和层析柱对该Mn-CAT进行纯化,酶学性质研究表明：此酶的最适温度为70℃,最适pH为pH 10.0,在80℃保温2h,酶活力不损失;pH9.0～11.0的环境中放置2h后,酶活仅损失约10％,此酶具有良好的工业开发潜力.
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