Abstract
The present work addresses the thermal remodelling of flexible plant viruses with a helical structure and virus-like particles (VLPs). Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions’ thermal transition into structurally modified spherical particles (SP) has been demonstrated. The work has established differences in formation conditions of SP from virions (SPV) and VLPs (SPVLP) that are in accordance with structural data (on AltMV virions and VLPs). SP originate from AltMV virions through an intermediate stage. However, the same intermediate stage was not detected during AltMV VLPs’ structural remodelling. According to the biochemical analysis, AltMV SPV consist of protein and do not include RNA. The structural characterisation of AltMV SPV/VLP by circular dichroism, intrinsic fluorescence spectroscopy and thioflavin T fluorescence assay has been performed. AltMV SPV/VLP adsorption properties and the availability of chemically reactive surface amino acids have been analysed. The revealed characteristics of AltMV SPV/VLP indicate that they could be applied as protein platforms for target molecules presentation and for the design of functionally active complexes.
Highlights
Alternanthera mosaic virus (AltMV) is a member of the genus Potexvirus, family Alphaflexiviridae [1]
Milli-Q water was used in the case of AltMV virus-like particles (VLPs) and SP from AltMV VLPs (SPVLP), 15 mM NaCl solution was used for SP from virions (SPV) and 12.5 mM Tris-HCl pH 8.0 was used in the case of AltMV virions for far-UV circular dichroism (CD) and intrinsic fluorescence measurements
It was demonstrated that the heating of AltMV virions under conditions suitable for PVX and TMV virions did not result in the generation of AltMV SPv [19]
Summary
Alternanthera mosaic virus (AltMV) is a member of the genus Potexvirus, family Alphaflexiviridae [1]. As we reported earlier [19], it is possible to obtain SP from AltMV VLPs (SPVLP) under conditions suitable for PVX and TMV SP formation. Milli-Q water was used in the case of AltMV VLPs and SPVLP, 15 mM NaCl solution was used for SPV and 12.5 mM Tris-HCl pH 8.0 was used in the case of AltMV virions for far-UV CD and intrinsic fluorescence measurements.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
