Abstract
Objective observe the therapeutic effects and to investigate the mechanisms of bone marrow derived mesenchymal stem cells(BMSCs) in treating chronic pancreatitis of rats. Methods BMSCs were isolated, cultured and amplified. Then, BMSCs were transfected with enhanced green fluorescent proteins combined with Ad5/F35 adenovirus vector. Chronic pancreatitis(CP) rat model was induced by infusion of oleic acid to bil-iopancreatic duct. Then, EGFP-BMSCs were transplanted to the CP rats through caudal vein injection for 3 times. Pancreatic tissues were collected and histopathological changes were observed under light microscope. Pancreatic CTGF,TGF-β,type-Ⅰ collagen, type-Ⅲ collagen and myeloperoxidase (MPO)activity were detected. The percentage of EGFP-labeled cells in the pancreatic tissue was examined by flow cytometer. Results The pathological injury and the fibrosis of BMSCs treated group were ameliorated significantly compared to those of CP group. The contents of pancreatic CTGF, TGF-β, type-Ⅰ collagen, type-Ⅲ collagen and MPO were all decreased obviously. The percentage of EGFP-labeled cells in the pancreatic tissue of BMSCs treated group was much higher than that of the negative control group. Conclusions BMSCs have obvious therapeutic effects in the treatment of CP, which may be related to their recruitments to the damaged pancreatic tissue as seed cells and their inhibition of CTGF,TGF-P release by autocrine or paracrine effects, thus decreasing the type-Ⅰ collagen, type-Ⅲ collagen and MPO producing. Key words: Bone marrow derived mesenchymal stem cells; Chronic pancreatitis; Fibrosis; Green fluorescent proteins; Flow cytometer
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