Abstract
In this article, biosurfactant production from low-cost and renewable substrate and its application in petroleum industry were studied. Pseudomonas aeruginosa DG30 grew on used vegetable oil and accumulated 15.56 g/L of biosurfactant at 72 hr. The biosurfactant exhibited excellent surface activity and stability. Sand package tests showed an approximately 20% increase of oil recovery by the treatment of bacterial culture broth. Single-well stimulation tests were conducted in a heavy oil reservoir in Dagang Oilfield, Tianjin, China. The blockages in the wellbore areas were removed effectively and more than 616 tons of crude oil was enhanced.
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