Abstract
A recent proposal in the Federal Register would prohibit the use of pooled reagent antibody screening red cells for pretransfusion compatibility testing within the United States. To evaluate the appropriateness of this proposal, 206 fresh serum samples known to contain potentially clinically significant red cell antibodies with Rh, Kidd, Kell, and/or Duffy blood group specificity were tested with pooled reagent antibody screening red cells; 9.7% of the sera that reacted with unpooled reagent antibody screening red blood cells failed to react with pooled screening cells. On the basis of these findings, pooled reagent antibody screening red blood cells should not be used for the pretransfusion compatibility testing of serum samples.
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