Abstract
Abstract. Bali cattle are one of Indonesian native cattle’s that famous for their ability to adapt to tropical harsh conditions. For the last 25 years, indiscriminate crossbreeding using artificial insemination (AI) organizing mainly by government might be has contaminated the purity of Bali cattle. In order to utilize as well as to conserve Bali cattle it is necessary to develop an accurate and simples method to detect the purity of Bali cattle. This experiment is the continuation of a long term experiments in developing methods to detect the purity of Bali cattle i.e. phenotypic variations, blood protein polymorphisms, hair structure, chromosome and DNA microsatellite variation analyses. The specificity of HEL9 and INRA035 microsetellites in Bali cattle as well as in Banteng (Bos sondaicus) as their ancestor was tested. The results show that A and B alleles at INRA035 microsatellite locus are monomorphic and can be used for a specific markers for Bali cattle. Allele A at locus HEL9 that has high frequency (92.90%) in Bali cattle and 100% in Banteng can also be used as a supporting marker.
Highlights
Bali cattle are one of several Indonesian native cattle that plays major role for providing meat
Due to indiscriminate crossbreeding, the pure Bali cattle at those areas have been contaminated with other breeds, i.e. Simmental, Limousine, Brangus, Charolais, indicating by high frequency of abnormal appearances, including abnormal colour patterns and horn shapes
Some samples that cannot be amplified by using HEL9 and INRA035 primers could indicate that the individuals do not have HEL9 and INRA035 alleles
Summary
Bali cattle are one of several Indonesian native cattle that plays major role for providing meat. Due to indiscriminate crossbreeding, the pure Bali cattle at those areas have been contaminated with other breeds, i.e. Simmental, Limousine, Brangus, Charolais, indicating by high frequency of abnormal appearances, including abnormal colour patterns and horn shapes. The methods for identifying the pure of Bali cattle has been developed and pioneered by NOOR et al (2000) They used iso-electrical focussing blood protein polymorphism analyses, DNA microsatellite analyses, chromosome structure analyses and hair structure analyses using scanning electron microscope. INRA035 locus in Bali cattle consistently has two alleles, so it is possible to use both loci as specific markers for Bali cattle Based on this finding, it is necessary to test the specific alleles using a larger sample at the major areas of Bali cattle distribution in Bali Island. It is expected that the results can be used to differentiate between the pure and un-pure Bali cattle
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