Abstract
Flow cell cytometry can be used in a sensitive fluorescent immunoassay to rapidly identify salmonellae of Groups D and E. Staphylococcal Protein A couples with the Fc region of many antibodies, especially immunoglobulin G. When a fluorescent marker, fluorescein isothiocyanate, is conjugated to Protein A, an antibody‐antigen reaction can be visualized in a flow cell cytometer with an appropriate optical sensor after excitation. Ten thousand cells were individually analyzed in each cytometer run within a minute. the resultant data, presented as histograms and tables, indicated the number of cells in the suspension reacting positively with the antiserum by fluorescence, cell size, distribution and light scatter. This provides an in‐depth analysis of the antibody‐antigen reaction not possible with other types of immunoassays.
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