The urinary excretion of aromatic acids by starved sheep.

Abstract

1. Four wether sheep were maintained on a diet of hay for 2 weeks and then starved for a period of 4 days.2. Immediately before and during starvation the urinary excretion in the following fractions was determined: hippuric acid, creatinine, total diethyl ether-soluble acids of hydrolysed and unhydrolysed urine, total aromatic acids in hydrolysed and unhydrolysed urine and the proportion of the former present as benzoic and phenylacetic acids.3. A method for determining the benzoic acid content of light petroleum extracts of urine has been developed and is described.4. Starvation had little effect on the urinary excretion of phenylacetic acid or creatinine, but during the first 2 days of starvation there were large decreases in the excretion of all the other urinary fractions studied.5. Of the fractions examined, 43% of the diethyl ether-soluble acids of hydrolysed urine and 42% of those of unhydrolysed urine were of exogenous origin; 76% of the total urinary aromatic acids were of exogenous origin. Partition of the aromatic acids in the urine of two of the four sheep indicated that the reduction in aromatic acid excretion on starvation was completely accounted for by the decline in benzoic acid output. Almost all the hippuric acid (97%) was of exogenous origin.6. These results have been compared with the urinary output of aromatic acids by nonruminants when fasted, and possible reasons for the relatively large amounts of phenylacetic acid found in the urine of starved sheep have been discussed.