Abstract
The lipids of intact Pseudomonas aeruginosa resting cells were extracted and the weights of the lipid fractions determined. The phospholipids were found to comprise a major portion (80%) of the ether-soluble lipids. Chromatograms of the phospholipid complex on silicic acid-impregnated paper revealed at least six fractions. Two-dimensional chromatograms of the aqueous acid hydrolyzate of the phospholipids indicated the presence of at least 13 ninhydrin positive compounds. There was a correlation between the uptake of dl -alanine-1-C14, dl -leucine-1-C14, and dl -phenylalanine-3-Cl4 by phospholipids of the resting cells and utilization of these amino acids by the cell as indicated by manometric studies.
Highlights
The lipids of intact Pseudomonas aeruginosa resting cells were extracted and the weights of the lipid fractions determined
An investigation was instigated to study the utilization of protein hydrolyzates and amino acids by P. aeruginosa in an attempt to develop better procedures for the identification of this gram-negative bacillus in the diagnostic laboratory
The ability of 18 different amino acids studied to stimulat,e O2 consumption of these cells ranged from 18.5 pl O2 uptake/W min/mg dry weight for m-methionine to 74.1 pl for m-serine
Summary
The lipids of intact Pseudomonas aeruginosa resting cells were extracted and the weights of the lipid fractions determined. The phospholipids were found to comprise a major portion (80%) of the ether-soluble lipids. Chromatograms of the phospholipid complex on silicic acid-impregnated paper revealed a t least six fractions. Two-dimensional chromatograms of the aqueous acid hydrolyzate of the phospholipids indicated the presence of a t least 13 ninhydrin positive compounds. There was a correlation between the uptake of ~ ~ - alanine - l - C~I ~b,leucine - l - C ~an~d, DL-phenylalanine-3-Cl4 by phospholipids of the resting cells and utilization of these amino acids by the cell as indicated by manomet.ric studies
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