The up-regulation of innate immune response can be observed by expression of TLR3, TLR4 and TLR9 by antigen-presenting cells in EAE model

Abstract

Event Abstract Back to Event The up-regulation of innate immune response can be observed by expression of TLR3, TLR4 and TLR9 by antigen-presenting cells in EAE model Marcilene G. Evangelista1, Sandra B. Castro1, Alyria T. Dias1, Caio Cesar Souza Alves1*, Maria A. Juliano2 and Ana Paula Ferreira1 1 Universidade Federal de Juiz de Fora, Parasitologia, Microbiologia e Imunologia, Brazil 2 Universidade Federal de Sao Paulo, Biofisica, Brazil Multiple sclerosis is a chronic inflammatory demyelinating disorder of the central nervous system. EAE is a model for the study of MS. The objective of this study was evaluating the innate immune response including Toll-like receptors in EAE model development. The EAE mice were immunized with MOG35–55, emulsified v/v in complete Freund’s adjuvant (CFA) supplemented with 400μg of attenuated Mycobacterium tuberculosis and Pertussis toxin 300ng/animal was injected intraperitoneally on the day of immunization and 48h later. The MOG negative group only received supplemented CFA and Pertussis toxin. The animals were euthanized at 2nd, 4th and 7th days post-immunization. The spinal cords were removed and analyzed. Only EAE group developed clinical signs. In comparison to the MOG negative group the level of CCL5 and CCL20 in homogenized spinal cord was higher in the EAE group at 4th (1,983.0±237.2 vs. 1,245.0±110.5 and 4,546.0±569.8 vs. 1,992.0±343.0) and 7th (1,459.0±97.3 vs. 717.6±110.0 and 3,262.0±331.4 vs. 1,762.0±259.6) days post-immunization. The levels of IL-10 (8,828.0±1,652.0 vs. 2,063.0±447.4) and TGF-β (128,139.0±55,265.0 vs. 14,311.0±5,461.0) were also higher in EAE group, which showed higher number of antigen-presenting cells expressing TLR3 (34,131.0±3,068.0 vs. 6,620.0±316.8), TLR4 (29,466.0±1,864.0 vs. 6,650.0±589.3) and TLR9 (104,346.0±4,407.0 vs. 21,626.0±1,434.0). The presence of MOG increases the release of chemokine in the spinal cord and up-regulation of TLRs expression by APC. Studies seeking the understanding of EAE may be important for the development of agents that modulate innate immune responses as signaling pathways TLRs may be effective in treating EAE and MS. Financial support: CAPES, CNPq, FAPEMIG. Keywords: EAE/MS, Toll-Like Receptors, APCs, innate immunity, IL-10, TGF-beta Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Evangelista MG, Castro SB, Dias AT, Souza Alves C, Juliano MA and Ferreira A (2013). The up-regulation of innate immune response can be observed by expression of TLR3, TLR4 and TLR9 by antigen-presenting cells in EAE model. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00008 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Caio Cesar Souza Alves, Universidade Federal de Juiz de Fora, Parasitologia, Microbiologia e Imunologia, Juiz de Fora, Minas Gerais, 36036900, Brazil, caiocsa@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marcilene G Evangelista Sandra B Castro Alyria T Dias Caio Cesar Souza Alves Maria A Juliano Ana Paula Ferreira Google Marcilene G Evangelista Sandra B Castro Alyria T Dias Caio Cesar Souza Alves Maria A Juliano Ana Paula Ferreira Google Scholar Marcilene G Evangelista Sandra B Castro Alyria T Dias Caio Cesar Souza Alves Maria A Juliano Ana Paula Ferreira PubMed Marcilene G Evangelista Sandra B Castro Alyria T Dias Caio Cesar Souza Alves Maria A Juliano Ana Paula Ferreira Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.