Abstract
The effects of ultrasonic radiation on cholesterol oxidase production by Brevibacterium sp. are studied in this paper. An ultrasonic wave with low intensity at 20 kHz, 200 W/ cm2 was employed to study the effects of irradiation at different lengths of time on the growth of Brevibacterium sp. cells. The result showed that the production of cholesterol oxidase from Brevibacterium sp. obviously increased, and was about 18.3%, when the culture was radiated for 1 min every 4 h. In order to investigate the action of ultrasonic on cell membrane and to facilitate enzyme excretion, transmission electron microscopy (TEM) was employed. Scanning of the bacterial cells by TEM also first revealed that holes appeared in the cytoplasmic region, then excluded from the cell barrage, and finally self-destructed with higher irradiating dosage.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
