Abstract
Summary Leucocytes absorb H-ions from weakly acid solutions. If the quantity of H-ions absorbed is great enough, the capacity of the leucocytes for phagocytosis is injured. In addition to the toxicity of the dissociated H-ions, lactic, acetic and butyric acids have a specific toxicity for leucocytes. The order of toxicity of the tested acids is: When phagocytic tests are carried out in vitro it is necessary to protect the leucocytes against the influence of acid solutions. This is accomplished by the use of a buffered saline solution. Some of the sodium citrate on the market is of an acid reaction and therefore is not suitable for use in the preparation of leucocytic suspensions for the tropin test. The union of the immune body (bacteriotropin) with the streptococcus was not influenced by such variations of H-ion concentration as were studied in these experiments.
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