Abstract

The Total Antioxidant Capacity Of Foods: A Reappraisal. Application To Commercial Orange Juices.

Highlights

  • Since a few years, more and more attention has been given to dietary antioxidants as agents promoting health and preventing the incidence of pathologies[1]

  • It is clear that the use of "total antioxidant capacity" assays for the in vitro assessment of antioxidant quality of food does not be employed by food industrials as a marketing argument or for the assessment of the "wholesomeness" of a food

  • Health benefits of fruit juices are attributed to a large number of compounds with biological activity; ascorbic acid, vitamin E, beta carotene, and phenolic compounds would be excellent antioxidants able to stabilize free radicals

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Summary

Introduction

More and more attention has been given to dietary antioxidants as agents promoting health and preventing the incidence of pathologies[1] As part of these efforts, analytical methods and assays have been developed to measure the antioxidant content in food substances. Additional methods have been developed like scavenging of superoxide anion generated by the xanthine/xanthine oxidase system[2], red blood cell resistance to oxidative stress (haemolysis) 3 or cellular antioxidant activity (CAA)[4] Among all these tests, the most publicized assay was the ORAC assay (Oxygen Radical Antioxidant Assay) initially developed two decades ago by scientists belonging to the National Institutes of Health and the U.S Department of Agriculture (USDA)[5,6]. In recent papers of us, we have, as an example, shown how analytical procedures (sample dilution, weight of analyzed sample, extraction conditions (nature of solvents mixture, time and temperature) could greatly influence the ORAC value[7,8]

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