Abstract
Background: Cancers are one of the leading causes of deaths nowadays. The development of new treatment schemes for oncological diseases is an interesting direction in experimental medicine. Therefore, the evaluation of the influence of two alkaloids—piperlongumine (PL), sanguinarine (SAN) and their combination—on the basic life processes of the A549 cell line was considered reasonable. Methods: The aim was achieved by analyzing the cytotoxic effects of PL and SAN and their combination in the ratio of 4:1 on the induction of cell death, changes in the distribution of cell cycle phases, reorganization of cytoskeleton and metastatic potential of A549 cells. The versatility of the applied concentration ratio was evaluated in terms of other cancer cell lines: MCF-7, H1299 and HepG2. Results: The results obtained from the MTT assay indicated that the interaction between the alkaloids depends on the concentration and type of cells. Additionally, the compounds and their combination did not exhibit a cytotoxic effect against normal cells. The combined effects of PL and SAN increased apoptosis and favored metastasis inhibition. Conclusion: Selected alkaloids exhibit a cytotoxic effect on A549 cells. In turn, treatment with the combination of PL and SAN in a 4:1 ratio indicates a synergistic effect and is associated with an increase in the level of reactive oxygen species (ROS).
Highlights
In addition to cardiovascular and cerebrovascular diseases, cancers are one of the leading causes of death
In order to confirm the results described above, the analysis was enriched with the assessment of the level of markers of the epithelial-to-mesenchymal transition (EMT), the process related to the metastatic potential of cells
We suggest that the treatment of A549 cells with a combination of PL and SAN in the ratio 4:1 indicates synergism without cytotoxic effects on normal lung cells
Summary
In addition to cardiovascular and cerebrovascular diseases, cancers are one of the leading causes of death. For patients with a late stage of cancer, metastasis are very often diagnosed. From a clinical point of view, the applied treatment procedure primarily depends on the type of lung cancer. In this context, two main groups may be distinguished: small-cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). The versatility of the applied concentration ratio was evaluated in terms of other cancer cell lines: MCF-7, H1299 and. Treatment with the combination of PL and SAN in a 4:1 ratio indicates a synergistic effect and is associated with an increase in the level of reactive oxygen species (ROS)
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