Abstract
Velocity sedimentation in sucrose density gradients has been used to separate and study the biologically active components of vesicular stomatitis (Indiana) grown in baby hamster kidney cells (VSV-BHK) and chick embryos (VSV-CE). If the main infective component in VSV-CE is given a sedimentation coefficient of 625 S, then another infective component of about 500 S was isolated by preferentially inactivating the main component with X-rays. The progeny from this after a single passage in BHK cells sedimented at about 750 S and continued to do so after many similar passages. The 750 S component was found to be more resistant to heat inactivation at 37° C than either the 625 S or 500 S components. The 625 S component was neutralized more efficiently with specific antiserum than either of the other two components. Both the 750 S and 500 S components had buoyant densities of 1.22 mg./ml. in CsCl.
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