The study of metabolic processes in the brain in conditions of hemorrhagic stroke on the background of pharmacotherapy with ademol

Abstract

In the article, on the model of subarachnoid hemorrhage, some biochemical aspects of the cerebroprotective effect of an industrial sample of an ampoule 1.0% solution of ademol are disclosed, namely its effect on carbohydrate and energy metabolism, the state of antioxidant systems, the activity of lipoperoxidation processes, and the functioning of the L-arginine / NO system in rat brain as possible metabolitotropic components of its protective effect on brain neurons. Severe subarachnoid hemorrhage was created under conditions of propofol anesthesia by injection of heparinized autologous blood (0.1 ml / kg volume) through a catheter located in the subarachnoid space. We used the Student t parametric criterion, W. White nonparametric criterion, Ť Wilcoxon paired criterion — to determine significant changes in the dynamics within the group. Differences were considered statistically significant at p<0.05. Using the example of the acute period of subarachnoid hemorrhage, it was established that ademol is able to eliminate brain energy deficiency (increase the content of adenosine triphosphoric acid and pyruvate in the brain while increasing the energy charge relative to the control pathology samples by an average of 45.1, 42.9 and 22.0%, p<0.05) reduce lactic acidosis (reduce the lactate content by 31.9%, p<0.05), eliminate the manifestations of oxidative stress (reduce the level of malondialdehyde and carbonyl groups of proteins on average by 30.5 and 18.8%, against the background of an increase in the activity of superoxide dismutase, glutathione peroxidase and catalase by 42.1, 25.2 and 37.6%, respectively, p<0.05), simulate the exchange of nitric monoxide (increase the activity of NO synthase with a simultaneous increase in the content of NO L-arginine donor on average by 14.0 and 44.0%, respectively, p<0.05). In these properties, ademol significantly exceeded the effectiveness of solutions of amantadine and magnesium sulfate.