The study of correlation between high-risk types HPV load and expression of nestin in different cervical lesions

Abstract

Objective To investigate the association between high-risk types of human papillomavirus (HR-HPV) load and the expression of nestin in different cervical lesions. Methods The hybridization captureⅡ(HC-Ⅱ) assay was used to test the HR-HPV load from 60 patients who were the first time to do the cervical cancer screening in Xiangya Hospital, and immunohistochemistry was used to detect the expression of nestin in those biopsy tissue samples. Results ⑴ The lgHPV level (logarithm of HR-HPV load) in the high level lesion group was higher than the low level one (P<0.05), and HR-HPV load was positively associated with the degree of cervical lesions (rs=0.269, P=0.037). ⑵ The expression of nestin in A group was weaker than groups B and C (H=7.271, 22.843, P<0.01), and the expression of nestin in C group was stronger than B group (H=7.270, P<0.01), and the expression of nestin was positively associated with the degree of cervical lesion (rs=0.646, P=0.000). ⑶ The HR-HPV load was positively associated with the expression of nestin (P<0.05). Conclusions The HR-HPV load and the expression of nestin are closely related to the cervical lesions, and the joint detection has a referential value for early prevention of cervical lesions and prediction of progress of cervical precancerous lesion. It might guide the early prevention and treatment of cervical cancers. Key words: Papillomaviridae/ME; Viral load; Intermediate filament proteins/ME; Nerve tissue proteins/ME; Uterine cervical diseases/ME