The study of bone repair effect of recombinant human bone morphogenetic protein-2 combined with artificial bone substitute materials in alveolar defect around the dental implant

Abstract

Objective To evaluate the bone repair capacity of β-tricalciumphosphate (β-TCP) or Bio-Oss combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in Beagle dogs.Methods Ten healthy adult Beagle dogs were used in this study.The mandibular premolars of the dogs were extracted firstly.Three months later,dental implants were placed in the mandibular,and a 5 mm× 4 mm× 3 mm bone defect area was prepared on the buccal side of each implant.The bone defect areas were filled with β-TCP,rhBMP-2/β-TCP compound,Bio-Oss or rhBMP-2/Bio-Oss compound respectively.The bone defect areas were filled nothing as the blank.The dogs were sacrificed after 4 or 12 weeks.Samples were prepared for the gross observation,X-ray analysis and histomorphology analysis.Results The new bone formation was observed in all experiment groups.After 4 weeks,the bone density of materials combined with rhBMP-2 groups [(36.70 ± 1.73) ％ and (38.50 ± 1.83) ％] were higher than the raw materials groups [(30.50 ± 1.81)％ and (31.48 ± 1.86)％].After 12 weeks,the bone density of materials combined with rhBMP-2 groups [(52.84 ± 1.85) ％ and (54.85 ± 1.87) ％] were also higher than the raw materials groups [(43.65 ± 1.81) ％ and (44.94 ± 1.88) ％].And new bone formation rate of the materials combined with rhBMP-2 groups were higher than the raw materials groups at each evaluation time.Conclusion rhBMP-2 can promote the bone regeneration activity of the artificial bone substitute materials effectively. Key words: Bone morphogenetic protein; Artificial bone substitute materials; Bone defect