Abstract

Piper sarmentosum and Piper nigrum which belongs to family Piperaceae are well distributed in the tropical region including Malaysia. They are one of the medicinal plants which are well known for its health benefits to human. This study focused on determining the antioxidant activity of P. sarmentosum and P. nigrum leaves of ethanolic and aqueous extraction. For the extraction, different concentrations of ethanol and aqueous extracts were used. For the antioxidant activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was conducted. The results showed that the scavenging activity of all extracts samples was in a concentration-dependent manner. High antioxidant activity of P. sarmentosum leaves was obtained by using ethanol extraction with 74% of inhibition, and IC50 value was 35.18 μg/mL. Meanwhile, P. nigrum leaves showed high antioxidant activity by using aqueous extraction with 64.68% of inhibition, and IC50 value was 79.89 μg/mL. It can be concluded that different extraction solvents used to give a different level of antioxidant activity of both P. sarmentosum and P. nigrum.

Highlights

  • Piper L. genus from Piperaceae family is economically important plants and beneficial for human health

  • Piper sarmentosum and Piper nigrum are among the plants which play an essential role as a medicinal plant

  • P. nigrum shows a higher percentage of inhibition compared to P. sarmentosum by using aqueous extraction

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Summary

Introduction

Piper L. genus from Piperaceae family is economically important plants and beneficial for human health. There are 1200 species of Piper distributed in the pantropical and Neotropical region in the world with more than 400 species have been recorded in Malaysia region alone (Rahman et al, 2016). Piper sarmentosum and Piper nigrum are among the plants which play an essential role as a medicinal plant. P. sarmentosum is a wild-growing plant in the tropical forests and mostly in Asia region. The mature leaves are simple, alternate and heart-shaped. The young leaves consist of waxy surface and light green in colour. P. nigrum is a woody perennial climbing vine that grows in the tropical area which leaves and fruit-producing fragrance and essential oil. The plant leaves are simple, alternate, oval-shaped and leathery in texture. The upper leaves surface is dark green and whitish-green on the underside

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