Abstract
In the past couple of decades, the nanopore field has used transmembrane proteins as scaffolds for functional biosensors. These sensors have proven highly sensitive and accurate. Yet, the engineering of nanopores requires a strenuous procedure to characterize their function. This sorting process has become more relevant, as the field expands to sensing without steric hindrance of the nanopore confinement. We address this problem by introducing a detailed procedure with key indicators to determine a nanopore’s functionality as a sensor before thorough testing.
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