The Sinergy of Isoquercetin and D-Galacturonic acid of Cyclea barbata Miers extract on the Migration and Apoptosis of Neutrophil in Rat Burn Wound

Abstract

Second-degree burn is one of acute traumas that has complexities in its healing process; one of which is the disruption of neutrophils activity and function as the initial cellular defense during inflammatory phase. Appropriate wound treatment can modulate the quantity of neutrophils in burn wounds. Cyclea barbata Miers (CBM) is a local plant that has a potential to influence inflammatory process. This study was aimed to determine the polyphenol and monosaccharide contents of CBM and the effect of CBM on neutrophils migration and apoptosis. We used 30 adult male Wistar rats as experimental animals, wich were randomly separated in two groups according the eutanized time. Each group divided in 3 groups (n=5 per group). Cyclea barbata Miers’ content was analyzed using liquid chromatography mass spectrometry (LCMS/MS) method, while neutrophils migration and apoptosis were observed using immunofluorescence assay method. The result of LCMS/MS assay showed that CBM contains isoquercetin (93.83ng/mL) and d-galacturonic acid (0.114ng/mL). Further result showed that CBM extract can decrease neutrophil migration and improve neutrophil apoptosis during the inflammatory phase of the burn wound. This ability may be enabled by the anti-inflammatory and antiradical properties of isoquercetin and DGA in Cyclea barbata Miers. Further study is required to confirm this hypothesis, specially addition of comparasion groups that treated by single compound such as isoquercetin and DGA.