Abstract
Heparin has been fractionated into two distinct forms. The isolation of these species was accomplished by sucrose density gradient centrifugation of heparin mixed with antithrombin-heparin cofactor. Approximately 1 3 of this mucopolysaccharide was bound to antithrombin-heparin cofactor and had potent anticoagulant activity. This component was clearly separated from the remaining 2 3 of the heparin which could not form a stable complex with antithrombin-heparin cofactor and had minimal anticoagulant activity.
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