Abstract
Chromatography on Sephadex gels can separate water-soluble azo and azomethine dye sulfunates from colored impurities that are not removed by recrystallization. The method gives sharp bands and high resolution; as many as eleven bands have been resolved from a crude azo dye preparation. Mixtures of isomeric dyes have been separated. The azomethine dyes studied here are separated from hydrolysis and oxidation products formed during the preparation, and from other similar dyes. The separations of both classes of dyes depend upon adsorption to the gels. Variations with gel cross-linking and the nature and concentration of electrolyte in the eluent are reported.
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