Abstract
Silk fibroin (SF) thin film was prepared via colloid chemical routes (CC) and by pulsed laser deposition (PLD). Secondary structure of the CC film, as examined by FT-IR, was random coil. By a post treatment with methanol, it transforms to β-sheet. As for PLD films, the β-sheet structure of SF powder was mostly preserved as deposited. This suggests that the post treatment is effective to restore the original β-sheet structure in the thin films. Transformation from random coil to β-sheet is easier in the film on the substrate of polyethylene than Si(1 0 0) due to weaker affinity to silk fibroin.
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