Abstract
Abstract Staphylococcus aureus is a common cause of mastitis, a disease estimated to cost the dairy industry ~$2 billion/yr. Public concern over the use of antibiotics in agricultural animals necessitates the identification of new mechanisms for controlling this pathogen. Our goal is to define the role of immune activating DC in the development of protective S. aureus-specific T cell memory and use this knowledge for identification of antigens for vaccine development. Our findings indicate IL-17 generated by PBMC in response to S. aureus loaded monocyte-derived DC that is greater in memory as compared to naïve animals. Lymphocytes stimulated with both live and gamma irradiated antigens presented by monocyte-derived DC released active Granzyme B. Cells from previously infected animals had higher Granzyme B response than animals with no previous infection. In animals with no previous clinical infections, irradiated S. aureus antigen induced lower response than live S. aureus. Furthermore, bovine neutrophils generate greater levels of cytokines when stimulated with supernatants from S. aureus induced T cells. This data indicates that previous infection can heighten the ability of cytotoxic T cells to produce Granzyme B and increase neutrophil function. The ability to increase presence of protective memory immune cells and innate immune cell function in the mammary gland will provide new vaccine targets for S. aureus.
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