Abstract
Uninduced microsomes from three different species of the genus Oncorhynchus catalyzed a NAD(P)H-dependent oxidation of the radical scavenging agent 2-keto-4-thiomethylbutyric acid (KMBA). This reaction was stimulated by the addition of iron-EDTA, hemoglobin and myoblobin but inhibited by catalase and benzoate. Superoxide dismutase (SOD) did not substantially inhibit the oxidation in the presence of iron-EDTA. This hydroxyl radical (·OH)-generating system was mainly NADH-dependent which is similar to observations in aquatic invertebrates but in contrast to mammalian systems. This study demonstrates that this radical-generating activity is wide ranging and requires consideration in evaluating xenobiotic metabolism.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
