Abstract
BackgroundThe purpose of this study is to investigate the possible attenuating role of the intra-articular administration of Fetuin-A in post-traumatic secondary osteoarthritis in rats, and also its effect on the systematic levels of interleukins (ILs)-2,4,7, bone morphogenetic proteins (BMPs) 2, 4, 7, C-Reactive Protein (CRP) and Fetuin-A.MethodsThirty male Sprague Dawley rats were separated in two groups where post-traumatic osteoarthritis was induced surgically by Anterior Cruciate Ligament Transection and the transection of the Medial Collateral Ligament of the right knee. In the Control Group, only the surgical intervention took place. In Fetuin Group, along with the induction of osteoarthritis, a single dose of bovine fetuin was administrated intra-articularly, intra-operatively. Both groups were examined for 8 weeks. The levels of interleukins, bone morphogenetic proteins, Fetuin-A and C-Reactive Protein were evaluated by ELISA of peripheral blood in three time periods: preoperatively, 5 and 8 weeks post-operatively. Osteoarthritic lesions of the knee were classified according to the Osteoarthritis Research Society International Grading System and the Modified Mankin Score, by histologic examination.ResultsIL-2 levels were significantly decreased in the Fetuin Group. No statistical difference was signed on the levels of IL-7, BMP-2,4,7 and Fetuin-A between the two groups. CRP levels were significantly increased in the Fetuin Group in 5 weeks of the experiment. Fetuin Group signed better scores according to the OARSI classification system and Modified Mankin Score, without any statistical significance.ConclusionsIntra-articular administration of Fetuin-A restrictively affected the progression of post-traumatic arthritis in rats, as only the levels of IL-2 were decreased as well as limited osteoarthritic lesions were observed on the Fetuin Group.
Highlights
The purpose of this study is to investigate the possible attenuating role of the intra-articular administration of Fetuin-A in post-traumatic secondary osteoarthritis in rats, and its effect on the systematic levels of interleukins (ILs)-2,4,7, bone morphogenetic proteins (BMPs) 2, 4, 7, C-Reactive Protein (CRP) and Fetuin-A
Since studies have shown that the invasive surgical protocols of Post-Traumatic Osteoarthritis (PTOA) induction in rat models are accompanied by the certain advantages above, we considered the most suitable animal model for the specific study could be the rat
Fetuin Group signed better scores according to the Osteoarthritis Cartilage Histopathology Assessment System (OARSI) classification system and the modified Mankin score system, in contrast to Control Group, no statistical significance was noted
Summary
The purpose of this study is to investigate the possible attenuating role of the intra-articular administration of Fetuin-A in post-traumatic secondary osteoarthritis in rats, and its effect on the systematic levels of interleukins (ILs)-2,4,7, bone morphogenetic proteins (BMPs) 2, 4, 7, C-Reactive Protein (CRP) and Fetuin-A. Fetuin-A was first declared as a fetus major plasma protein and is described as alpha-2-HS-glycoprotein (AHSG). It is expressed in the liver, kidney, gastrointestinal tract, skin and brain, during the fetal growth (Pappa et al 2017). Fetuin-A drew interest regarding its possible protective role against inflammation in injury or infection, being classified as a positive APP thanks to the mediation of High Mobility Group protein (HMGB1), and as a negative APP because of a regulation of proinflammatory cytokines such as tumor necrosis factor (TNF), interleukins (IL) -1, IL-6, IL-12, IL-18 and interferon (IFN)-γ (Sluijs et al 1992; Kraus et al 2011). Wang et al in 2010 proved the protective role of Fetuin-A in the ischemic cerebral inflammation in rat models together with the suppression of sepsis mediators in late stages of sepsis in the same animal model of rats (Wang and Sama 2012)
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