Abstract
In a recent study (3), changes in contents of y-aminobutyric acid were associated with cold hardening and dehardening patterns of crowns of Pawnee winter wheat during each of 2 seasons. Perkins and Andrews (2) found that survival from freezing of sprouting wheat seeds of a cold hardy and a nonhardy variety was significantly increased by adding a 0.25M solution of y-amino-n-butyric acid to the growth media 24 hours before freezing. These results suggested the desir'ability of comparing the effects of supplemental feeding on hardening characteristics of sprouting winter wheat seeds with changes in y-aminobutyric acid contents of crowns of older plants of the same varieties during cold hardening and dehardening under field conditions.
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