The Research of HPAM Degradation Efficiency of an Anaerobic Strain SN10 with Simultaneous Capabilities of Denitrification and Sulfate Reduction

Abstract

Anaerobic bacterium (strain SN10) with simultaneous capabilities of denitrification and sulfate reduction was enriched and isolated by anaerobic Hungate technology. The characterisation of SN10 was conducted from the morphologial, physiological, phylogenetic aspects and HPAM degradation efficiency. It was a rod-shaped, gram-negative, 0.40~1.0μm in width and 1.5~2.5 μm in length. Most of the fatty acids of SN10 was distributed among C12:0~C19-CYC-FAME and the main of them was consisted of C14:0 FAME, C16:0-FAME, C18:1c-FAME, C18:0-FAME and C19-CYC-FAME. Based on the morphologial, physiological characteristics and the phylogenetic analysis, SN10 was identified as Paenibacillus sp. (EU124565), Because its 16S rDNA can reach 97% of the similarity to that of Paenibacillus lautus SN10 (Accession No.DQ450463). The removal efficiencies of NO3 - and SO4 2- were up to 98.52% and 96.49 % when the initial concentrations of NO3 - and SO4 2- were 5830.92 mg/L and 682.83 mg/L, HPAM solution density was 2400mg/L, three days strain biodegradation rate was 68.27%, which can result in obviously decreased viscosity. The SN10 strain isolated would have provided the new microorganism resources for the polyacrylamide biodegradation.