Abstract

Abstract Strains of siderophore producing Pseudomonas sp. were isolated from the rhizospheres of cotton (Gossypium herbaceum L.) and peanuts (Arachis hypogaea) (strains 1 and 11, respectively). The siderophores were produced in iron deficient culture medium. FeCl3 was added to the culture media at the end of the growth period in order to produce an iron pigment complex. These solutions were applied to the soil directly or after removal of bacteria and free salts by centrifugation and dialysis. Peanuts grown in 0.5 kg pots on a highly calcareous soil (63% CaCO3) and exhibiting severe iron chlorosis were used as test plants to the iron‐siderophore or iron‐siderophore‐bacteria activity as soil amendment for iron deficiency. The plants were treated with (1) FeEDDHAM10 mg 500 g‐1 soil; (2) iron pigment complex of the two isolates; and (3) bacterial suspension containing the iron pigment complex. Plants grown on untreated soil were used as a control. Significant increase of chlorophyll concentration of th...

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