Abstract
Rabbits were immunized with highly conjugated dinitrophenylated coliphage MS2 containing 150 DNP groups per phage particle that were purified by isopycninc CsGl gradient centrifugation. The anti-DNP 150MS2 IgG was analyzed over the course of immunization in terms of its anti-hapten, anti-carrier and anti-hapten-carrier reactivity. Neutralization by unabsorbed as well as MS2 and DNP-agarose absorbed IgH revealed that the dominant reactivities were carrier or hapten-carrier specific rather than hapten specific. Subsequent work with DNP-conjugated MS2 coat protein monomers indicated the role that extent of conjugation, accessibility of the haptenic group and carrier complexity play in determining the relative immunodominance o DNP haptenic groups on MS2.
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