Abstract
Five rat tissues, liver, kidney, heart, brain, and testis, metabolize L-[1-14C]leucine by two pathways. The predominant flux is through the alpha-keto pathway in all tissues; the flux in the beta-keto pathway is approximately 1 to 5% of the total flux for all tissues except the testis where the flux in the beta-keto is one-third of the total flux. Intrinsic factor, which binds the cobalamin cofactor of leucine 2,3-aminomutase, effectively blocked the beta-keto pathway.
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