Abstract
An improved method for the reconstitution of DNA-dependent RNA polymerase from its isolated subunits in the absence of urea is described. It allows the kinetic analysis of the assembly mechanism. The rate of reconstitution as well as the final yield are strongly temperature-dependent, whereas concentration has only a small effect. The kinetics of the formation of an intermediary complex α2β and an inactive enzyme complex α2ββ′σ were followed. The rate-limiting and temperature-sensitive step during reconstitution is the conversion of the inactive complex α2ββ′σ into active enzyme.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
