Abstract
To investigate possible protective effect of the polyphenol concentrate from Cabernet Sauvignon collection of grapes in rat model of acute radiation sickness (ARS). To initiate the condition (ARS) 34 white outbred male rats weighing 160±20 g were exposed to the total acute gamma-irradiation of 8.0 Gy. The progress of radiation sickness was monitored by hematological blood tests one week after irradiation. Animals were divided into 4 groups: group 1, where animals received the polyphenol concentrate the week before and the week after irradiation, group 2 - received the polyphenol concentrate only before irradiation, group 3 - received the polyphenol concentrate only after irradiation, group 4 – control – none of the animals received the polyphenol concentrate. Experimental animals were given 0.5 ml of the polyphenol concentrate intragactrically on daily basis. The level of oxidative stress in the blood plasma was studied with FRAS 4 (Evolvo srl, Italy) using sets of d-ROMs test (shows the amount of free radicals in plasma) and PAT Test (evaluates the total antioxidant activity of blood plasma). In comparison to intact animals all irradiated animal developed pancytopenia. Erythrocyte indicators such as red blood cell count, hemoglobin, and hematocrit were also decreased. Group 1 and group 3 showed less decrease in hemoglobin, platelets and erythrocytes in blood compared to other groups. Activation of the oxidative stress (d-ROMs Test) and reduction of the systemic antioxidant potential (PAT Test) was observed in all groups of irradiated animals. It was also shown that the polyphenol concentrate prevented the reduction of the total antioxidant activity of blood plasma in all treated groups (groups 1, 2 and 3) in comparison to untreated animals (group 4). The polyphenol concentrate prevents reduction of the total antioxidant activity of plasma, as well as limits the progress of pancytopenia.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
